Part:BBa_K3670000
XplA/B play a key role in the biodegradation of RDX.
The xplA and xplB gene fusion are involved in the reductive denitrification and ring cleavage of explosive organic contaminant, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). The xplA/B system are normally located adjacent to each other in a plasmid-borne gene operon from Rhodococcus rhodochrous strain 11Y.
First, the xplB gene encodes for a partner flavodoxin reductase, while the xplA encodes for flavodoxin domain fused (at the N-terminus) of a P450 cytochrome. Flavodoxins are small electron transfer proteins containing one molecule of non-covalently, but tightly bound flavin mononucleotide (FMN) as its redox center. The partner flavodoxin reductase, encoded by the xplB gene, is required to complete the system and it's related to the redox partner of xplA. The reductase xplB facilitates the electron transfer from NADPH to the flavodoxin domain of the xplA cytochrome, which will ultimately result in the degradation of RDX by xplA. Depending on anaerobic or aerobic conditions, RDX in the form of MEDINA and 4-nitro-2,4, diazabutana (NDAB) respectively, produce nitrite and formaldehyde as byproducts. On the other hand, under aerobic condition, 4-nitro-2,4, diazabutanal (NDAB), nitrite and formaldehyde are produced.
Mathematical Models
To model the reactions that take place during the transcription of xplA/B, Team iGEM RUM-UPRM2020 used the Law of Mass Action kinetics to determine how the concentrations of the species changes with respect to time. In Figures 2 and 3, provides the ODE diagram representation in SimBiology and Concentration (mM) versus time (hr) plots to describe the system’s behavior. An overall analysis for all devices shows similar behaviors of the concentration as a function of time, as can be observed, the time is directly proportional to the concentration of translated proteins. As the time increases, the concentration of translated proteins increases.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 936
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Illegal PstI site found at 2775 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 936
Illegal EcoRI site found at 1847
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Illegal PstI site found at 1714
Illegal PstI site found at 2775 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 936
Illegal EcoRI site found at 1847
Illegal EcoRI site found at 2288
Illegal BglII site found at 1897
Illegal BamHI site found at 2281
Illegal BamHI site found at 2485
Illegal XhoI site found at 2819 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 936
Illegal EcoRI site found at 1847
Illegal EcoRI site found at 2288
Illegal PstI site found at 1714
Illegal PstI site found at 2775 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 936
Illegal EcoRI site found at 1847
Illegal EcoRI site found at 2288
Illegal PstI site found at 1714
Illegal PstI site found at 2775
Illegal NgoMIV site found at 148
Illegal NgoMIV site found at 840
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Illegal NgoMIV site found at 1957
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Illegal NgoMIV site found at 2158
Illegal NgoMIV site found at 2511
Illegal AgeI site found at 942 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 751
Illegal BsaI.rc site found at 1796
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